אודות
Highly regenerative tissues such as blood, skin, gut and testis are maintained by rare populations of tissue specific stem cells. Throughout the life of the organism stem cells replace cells spent to normal turnover and repair. Unlike their embryonic counterparts, tissue stem cells are already committed to their lineage and must achieve a perfect balance with differentiated progeny. A major control-unit that regulates this balance is the stem cell microenvironment, known as the niche. In our lab we focus on stem cell – niche communication and on mechanisms that maintain such unique cell compartments. We are mainly interested in post-transcriptional mechanisms as regulation at the mRNA levels enables the dynamics required for continuous stem cell activity. The highly regenerative Drosophila testis and S2 culture cells are the main research systems in our lab.
פרסומים
- Notch is a ligand of Draper mediating spermatogonia cell death, Abo Romi, H., Serhan, N., Porat-Kuperstein, L. & Toledano, H., Dec 2026, In: Nature Communications. 17, 1, 6003.
- Steady and escort cyst cells regulate Drosophila germline stem cell differentiation and death, Ben-Hamo-arad, A., Serhan, N., Porat-Kuperstein, L., Shklyar, B., Simchi, L., Kolpakova, A., Arama, E. & Toledano, H., 2 Feb 2026, In: Journal of Cell Biology. 225, 2, e202501134.
- Integrin restriction by miR-34 protects germline progenitors from cell death during aging, Perry, N., Braun, R., Ben-Hamo-Arad, A., Kanaan, D., Arad, T., Porat-Kuperstein, L. & Toledano, H., Jun 2024, In: Aging Cell. 23, 6, e14131.
- Sedentary Behavior Impacts on the Epigenome and Transcriptome: Lessons from Muscle Inactivation in Drosophila Larvae, Brener, A., Lorber, D., Reuveny, A., Toledano, H., Porat-Kuperstein, L., Lebenthal, Y., Weizman, E., Olender, T. & Volk, T., Oct 2023, In: Cells. 12, 19, 2333.
- Live Imaging of Phagoptosis in ex vivo Drosophila Testis, Kanaan, D., Shklyar, B., Porat-Kuperstein, L. & Toledano, H., 20 Mar 2023, In: Bio-protocol. 13, 6, e4637.
- The phagocytic cyst cells in Drosophila testis eliminate germ cell progenitors via phagoptosis, Zohar-Fux, M., Ben-Hamo-Arad, A., Arad, T., Volin, M., Shklyar, B., Hakim-Mishnaevski, K., Porat-Kuperstein, L., Kurant, E. & Toledano, H., Jun 2022, In: Science Advances. 8, 24, eabm4937.
- microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis, Volin, M., Zohar-Fux, M., Gonen, O., Porat-Kuperstein, L. & Toledano, H., 1 Nov 2018, In: Journal of Cell Biology. 217, 11, p. 3829-3838 10 p.
- MiR-9a modulates maintenance and ageing of Drosophila germline stem cells by limiting N-cadherin expression, Epstein, Y., Perry, N., Volin, M., Zohar-Fux, M., Braun, R., Porat-Kuperstein, L. & Toledano, H., 1 Dec 2017, In: Nature Communications. 8, 1, 600.
- MicroRNAs in Drosophila regulate cell fate by repressing single mRNA targets, Perry, N., Volin, M. & Toledano, H., 2017, In: International Journal of Developmental Biology. 61, 3-5, p. 165-170 6 p.
- Age-related changes to Drosophila m. male germline stem cells, Toledano, H. & Jones, D. L., 1 Jan 2015, Stem Cell Aging: Mechanisms, Consequences, Rejuvenation. Springer-Verlag Vienna, p. 71-84 14 p.
- Why adult stem cell functionality declines with age? Studies from the fruit fly drosophila melanogaster model organism, Gonen, O. & Toledano, H., 1 Sep 2014, In: Current Genomics. 15, 3, p. 231-236 6 p.
- Homothorax plays autonomous and nonautonomous roles in proximodistal axis formation and migration of the Drosophila renal tubules, Zohar-Stoopel, A., Gonen, N., Mahroum, M., Ben-Zvi, D. S., Toledano, H. & Salzberg, A., Jan 2014, In: Developmental Dynamics. 243, 1, p. 132-144 13 p.
- The role of the heterochronic microRNA let-7 in the progression of aging, Toledano, H., Jul 2013, In: Experimental Gerontology. 48, 7, p. 667-670 4 p.
- Dual fluorescence detection of protein and RNA in Drosophila tissues, Toledano, H., D'Alterio, C., Loza-Coll, M. & Jones, D. L., 6 Sep 2012, In: Nature Protocols. 7, 10, p. 1808-1817 10 p.
- The let-7-Imp axis regulates ageing of the Drosophila testis stem-cell niche, Toledano, H., D'Alterio, C., Czech, B., Levine, E. & Jones, D. L., 2012, In: Nature. 485, 7400, p. 605-610 6 p.
- Mechanisms regulating maintenance of stem cells and the stem cell niche., Toledano, H., D'Alterio, C., Czech, B., Levine, E. & Jones, D. L., 2011, p. 82-83. 2 p.
- Mechanisms regulating stem cell polarity and the specification of asymmetric divisions, Toledano, H. & Jones, D. L., 2009, StemBook. Cowan, C. A. & Melton, D. (eds.). IOS Press, p. 7
- Tissue development and RNA control: "HOW" is it coordinated?, Volk, T., Israeli, D., Nir, R. & Toledano-Katchalski, H., Feb 2008, In: Trends in Genetics. 24, 2, p. 94-101 8 p.
- Post-transcriptional repression of the Drosophila midkine and pleiotrophin homolog miple by HOW is essential for correct mesoderm spreading, Toledano-Katchalski, H., Nir, R., Volohonsky, G. & Volk, T., Oct 2007, In: Development (Cambridge). 134, 19, p. 3473-3481 9 p.
- Cell divisions in the Drosophila embryonic mesoderm are repressed via posttranscriptional regulation of string/cdc25 by HOW, Nabel-Rosen, H., Toledano-Katchalski, H., Volohonsky, G. & Volk, T., 22 Feb 2005, In: Current Biology. 15, 4, p. 295-302 8 p.
- Dimerization in vivo and inhibition of the nonreceptor form of protein tyrosine phosphatase epsilon, Toledano-Katchalski, H., Tiran, Z., Sines, T., Shani, G., Granot-Attas, S., Den Hertog, J. & Elson, A., Aug 2003, In: Molecular and Cellular Biology. 23, 15, p. 5460-5471 12 p.
- Protein tyrosine phosphatase ε inhibits signaling by mitogen-activated protein kinases, Toledano-Katchalski, H., Kraut, J., Sines, T., Granot-Attas, S., Shohat, G., Gil-Henn, H., Yung, Y. & Elson, A., 1 May 2003, In: Molecular Cancer Research. 1, 7, p. 541-550 10 p.
- Nuclear localization of non-receptor protein tyrosine phosphatase ε is regulated by its unique N-terminal domain, Kraut, J., Volohonsky, G., Toledano-Katchalski, H. & Elson, A., 2002, In: Experimental Cell Research. 281, 2, p. 182-189 8 p.
- Generation of novel cytoplasmic forms of protein tyrosine phosphatase epsilon by proteolytic processing and translational control, Gil-Henn, H., Volohonsky, G., Toledano-Katchalski, H., Gandre, S. & Elson, A., 7 Sep 2000, In: Oncogene. 19, 38, p. 4375-4384 10 p.
- The transmembranal and cytoplasmic forms of protein tyrosine phosphatase epsilon physically associate with the adaptor molecule Grb2, Toledano-Katchalski, H. & Elson, A., 9 Sep 1999, In: Oncogene. 18, 36, p. 5024-5031 8 p.
- Photodynamic damage by liposome-bound porphycenes: Comparison between in vitro and in vivo models, Toledano, H., Edrei, R. & Kimel, S., Jan 1998, In: Journal of Photochemistry and Photobiology B: Biology. 42, 1, p. 20-27 8 p.