The Flow Cytometry Unit contains a FACS CantoII analyzer, a Sony MA900 cell sorter and a work station with the flow analysis software, FlowJo.
All users receive training and guided assistance.
FACS CantoII Analyzer:
The BD FACS CantoII system is an automated benchtop flow cytometry system equipped with three lasers: blue (488-nm, air-cooled, 20-mW solid state), red (633-nm, 17-mW HeNe), and violet (405-nm, 30-mW solid state). It can be used to simultaneously analyze up to 8 fluorescent parameters and 2 scatters.
The analyzer is equipped with the BD FACS Loader that allows walkaway sample introduction to further improve productivity. The BD FACS Loader carousel accommodates up to 40 12×75-mm tubes and automatically loads them on the BD FACSCanto II system without operator intervention.
Applications:
Immunophenotyping
Apoptosis and Cell Viability
Transfection efficiency
Cell Cycle and DNA Content
Cell Signaling
Calcium flux
Cell proliferation